Product Name :
CDX2 monoclonal antibody Background :
CDX2, a homeobox domain-containing transcription factor, is a master regulator of the trophectoderm, the layer that gives rise to extra-embryonic tissues in mammalian development . CDX2 is also involved in intestinal development, and gain of expression or loss of expression has been associated with various human malignancies, such as Barrett's esophagus and colorectal cancer . Mouse embryonic stem cells deficient in CDX2 display limited hematopoietic progenitor development and altered Hox gene expression , pointing to a role for CDX2 in Hox gene regulation. CDX2 is also implicated in the aberrant expression of Hox genes in human AML cell lines . Product :
Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of CDX2 protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within human CDX2. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification

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  Immunohistochemical analysis of CDX2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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