Product Name :
MUC6 monoclonal antibody Background :
Mucin 6 (also designated MUC 6 and gastric mucin) is a large glycoprotein that plays a major role in the protection of the gastrointestinal tract. Mucin 6 carries GlcNAca1Æ4GalβÆR structures, indicating that a1,4-N-acetylglucosaminyltransferase is important to the formation of the mucous glycoproteins in vivo. Mucin 6 expression is highest in the stomach and gall bladder, with lower expression in the terminal ileum and right colon. In gastric cancer, Mucin 6 has an altered expression. In normal stomach, Mucin 6 apomucin is associated with Lewis type 2; Mucin 6 is also expressed in gastric metaplasia, duodenum and pancreas. Mucin 6 is a secretory mucin, located in the deeper mucosal folds of human gall bladder, and its expression is altered with increasing degrees of inflammation. Mucin 6 mRNA is expressed transiently in the nephrogenic zone of the kidney in the early mid-trimester of development, and Mucin 6 glycoprotein is expressed in the epithelium of ureteric buds and collecting ducts, but absent from adult kidney. Proliferating bile ductular cells express Mucin 6 apomucin in diseased liver, especially in chronic viral hepatitis with active necroinflammation, suggesting that this secreted mucin acts as a cytoprotective agent and represents a phenotype of reactive biliary epithelium in chronic viral hepatitis. Product :
Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of MUC6 protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within human MUC6. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification

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  Immunohistochemical analysis of MUC6 staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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