Product Name :
ALK5 monoclonal antibody Background :
A total of three members of the TGFb family, namely TGFb1, TGFb2 and TGFb3, have been identified in mammals. Each is synthesized as a latent precursor that is subsequently cleaved forming the 112 amino acid growth factor which becomes active upon dimerization. TGFbs mediate their activity by high affinity binding to the TGFb receptor type-II (TGFb RII) with a cytoplasmic serine-threonine kinase domain. For signaling growth inhibition and early gene responses, TGFb RII requires both its kinase activity and its association with a TGFb-binding protein, designated TGFb receptor type-1 (TGFb RI). TGFb RI is a 503 amino acid single-pass type I membrane protein that is expressed ubiquitously and, with TGFb RII, functions as a receptor for TGFb. Defects in the gene encoding TGFb RI are the cause of aortic aneurysm familial thoracic type 5 (AAT5), Loeys-Dietz syndrome type 2A (LDS2A) and Loeys-Dietz syndrome type 1A (LDS1A). Product :
Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of ALK5 protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within human ALK5. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification

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  Immunohistochemical analysis of ALK5 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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