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Histone H2A.Z (AcK13) polyclonal antibody BS67178
  • Western blot analysis of Histone H2A.Z (AcK13) expression in zebrafish (A) whole cell lysates.
Product NameHistone H2A.Z (AcK13) polyclonal antibody
Catalog No.BS67178
Swiss-ProtP0C0S5
Host Rabbit
ReactivityHuman, Zebrafish
ApplicationsWB
Application_allWB (1/500 - 1/1000)
BiowMW~ 13 kDa
Alternative NameH2AZ; Histone H2A.Z; H2A/z
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 139% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
Histone H2A.Z (AcK13) polyclonal antibody
Background :
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Product :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Recognizes endogenous levels of Histone H2A.Z with an acetylation site at K13 protein.
Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H2A.Z with an acetylation site at K13. The exact sequence is proprietary.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of Histone H2A.Z (AcK13) expression in zebrafish (A) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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