Product Name :
RORγ polyclonal antibody Background :
The nuclear orphan receptors RORα and RORg are members of the nuclear hormone receptor superfamily. Members of this family acts by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORα and RORg are unique in that they bind DNA as monomers. RORα has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. RORg comprises a 560 amino acid protein that shares 50% amino acid identity with RORα and is most highly expressed in skeletal muscle. Although these proteins are considered “orphan receptors,” due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors. The gene encoding RORα maps to chromosome 15q22.2 and the gene encoding RORg maps to chromosome 1q21.3. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RORγ polyclonal antibody detects endogenous levels of RORγ protein. Immunogen :
Synthetic peptide, corresponding to Human RORγ. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of RORγ polyclonal antibody at 1:1000 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug)
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  Immunohistochemistry of paraffin-embedded Human Brain using RORγ antibody at dilution of 1:50.
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  Immunohistochemistry of paraffin-embedded Human Brain using RORγ antibody at dilution of 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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