Product Name :
ATG9B (G883) polyclonal antibody Background :
Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. In addition to autophagy, also plays a role in necrotic cell death. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ATG9B (G883) polyclonal antibody detects endogenous levels of ATG9B protein. Immunogen :
Synthetic peptide, corresponding to Human ATG9B. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of ATG9B (G883) polyclonal antibody at 1:5000 dilution Lane1:C6 whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug) Lane3:The Brain tissue lysate of Rat(40ug) Lane4:The Brain tissue lysate of Mouse(40ug) Lane5:MCF-7 whole cell lysate(40ug) Lane6:HepG2 whole cell lysate(40ug) Lane7:Jurkat whole cell lysate(40ug)
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  Immunoprecipitation of HEK293T cell lysates using ATG9B (G883) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1) .Lane 4 is 30% input. The western blot was probed using ATG9B (G883) pAb #AP0781.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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