NFkB-p65 (G530) polyclonal antibody 
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Product Name :
NFkB-p65 (G530) polyclonal antibody Background :
p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory prd in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibittransforming activity. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NFκB-p65 (G530) polyclonal antibody detects endogenous levels of NFκB-p65 protein. Immunogen :
Synthetic peptide, corresponding to Human NFkB-p65. Conjugate :
Unconjugated Modification :
Unmodification
NFkB-p65 (G530) polyclonal antibody Background :
p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory prd in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibittransforming activity. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NFκB-p65 (G530) polyclonal antibody detects endogenous levels of NFκB-p65 protein. Immunogen :
Synthetic peptide, corresponding to Human NFkB-p65. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of NfkB-p65 pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:CT-26 whole cell lysate(40ug) -
Immunoprecipitation of MCF-7 cell lysates using NFkB-p65 (G530) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using NFkB-p65 (G530) pAb #BS1257. -
Immunoprecipitation of MCF-7 cell lysates using NFkB-p65 (G530) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using NFkB-p65 (G530) pAb #BS1257. -
Immunoprecipitation of MCF-7 cell lysates using NFkB-p65 (G530) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using NFkB-p65 (G530) pAb #BS1257.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.