Product Name :
BMP-5 (L163) polyclonal antibody Background :
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cartilage and bone formation or neurogenesis.Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2.In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes.Can also signal through non-canonical pathway such as MAPK p38 signaling cascade to promote chondrogenic differentiation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
BMP-5 (L163) polyclonal antibody detects endogenous levels of BMP-5 protein. Immunogen :
Synthetic peptide, corresponding to Human BMP-5. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of BMP-5 (L163)polyclonal antibody at 1:2000 dilution Lane1:EC9706 whole cell lysate(30ug) Lane2:HCT116 whole cell lysate(30ug) Lane3:A549 whole cell lysate(30ug) Lane4:HEK293T whole cell lysate(30ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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