Product Name :
SLC25A5 polyclonal antibody Background :
Adenine nucleotide translocase 2 (ANT2/SLC25A5) is a member of the adenine nucleotide translocase family of mitochondrial inner membrane proteins that function differently in metabolic and apoptotic pathways. Research studies indicate that ANT2 expression in undifferentiated, proliferating cells correlates with the rate of glycolytic metabolism and may be an indicator of carcinogenesis. Suppression of ANT2/SLC25A5 expression by specific RNA interference in human breast cancer cells promotes apoptosis and inhibits tumor cell growth, which suggests a cytoprotective role of ANT2/SLC25A5. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SLC25A5 polyclonal antibody detects endogenous levels of SLC25A5 protein. Immunogen :
Synthetic peptide, corresponding to Human SLC25A5. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of SLC25A5 polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate(30ug) Lane2:HEK293T whole cell lysate(30ug) Lane3:SGC7901 whole cell lysate(30ug) Lane4:CT-26 whole cell lysate(30ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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