Product Name :
TNAP Rabbit monoclonal antibody Background :
Alkaline phosphatases (AP) are glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallated glycoproteins that catalyze the hydrolysis of phospho-monoesters into an inorganic phosphate and an alcohol. There are at least four distinct but related alkaline phosphatases: intestinal (IAP), placental (PLAP), placental-like (ALP-1 or GCAP) and tissue non-specific (TNAP). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. TNAP is widely expressed in liver, kidney, bone, stomach and colon, and is therefore referred to as the tissue non-specific form of AP. TNAP, in conjuntion with plasma cell membrane glycoprotein-1, function in bone mineralization; however, mice that lack a functional form of TNAP show normal skeletal development. Product :
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TNAP monoclonal antibody detects endogenous levels of TNAP protein. Immunogen :
KLH conjugated synthetic peptide derived from human Alkaline Phosphatase: 18-50 Conjugate :
Unconjugated Modification :
Unmodification

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  Primary: Anti-Alkaline Phosphatase at 1/1000 dilution
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  Tissue: Human liver Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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