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AMPK beta 1 monoclonal antibody MB67259
  • Western blot analysis of AMPK beta 1 expression in C2C12 (A), H4IIE (B), NIH3T3 (C), PC12 (D) whole cell lysates.
Product NameAMPK beta 1 monoclonal antibody
Catalog No.MB67259
Swiss-ProtQ9R078/P80386
Host Mouse
ReactivityMouse, Rat
ApplicationsWB
Application_allWB (1/500 - 1/1000)
BiowMW~ 40 kDa
Alternative NameAMPK; 5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb
Purification&PurityThis antibody is purified through a protein G column.
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
AMPK beta 1 monoclonal antibody
Background :
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) (By similarity).
Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Recognizes endogenous levels of AMPK beta 1 protein.
Immunogen :
Recombinant fusion protein of human AMPK beta 1. The exact sequence is proprietary.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of AMPK beta 1 expression in C2C12 (A), H4IIE (B), NIH3T3 (C), PC12 (D) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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