Product Name :
PURB monoclonal antibody Background :
Purβ (purine-rich element-binding protein B), also known as transcriptional activator protein Purβ, is a 312 amino acid protein that belongs to the PUR DNA-binding protein family. The Purβ gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, the Purβ protein is implicated in the control of both DNA replication and transcription. Deletion of the Purβ gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia (AML), which is a malignant disease where in hematopoietic precursors are arrested in an early stage of development. Localizing to nucleus, the Purβ protein is expressed in myocardium of heart failure patients. The Purβ gene is conserved in mouse, rat, zebrafish, fruit fly, mosquito, C. elegans, A. thaliana and rice. and maps to human chromosome 7p13. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of PURB protein. Immunogen :
Recombinant fusion protein of human PURB. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of PURB expression in Jurkat (A), HeLa (B), NCIH460 (C), HepG2 (D), MCF7 (E), NIH3T3 (F) whole cell lysates.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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