XRN1 monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
XRN1 monoclonal antibody Background :
5’-3’ exoribonuclease 1 (XRN1) is a cytoplasmic exonuclease that degrades RNA containing a 5’-monophosphate to component mononucleotides. XRN1 is the primary exonuclease associated with ribosomes in the cytoplasm and is responsible for mRNA turnover. This turnover is facilitated in discrete structures in the cytoplasm called P-bodies that contain decapping and deadenylation proteins. XRN1 also plays a role in RISC-mediated mRNA degradation, as it associates with 3’ mRNA fragments generated by RISC cleavage. This process does not require uncapping or deadenylation. XRN1 plays a significant role in viral RNA degradation. As such, many viral genomes, including hepatitis C, Dengue, and West Nile, encode for XRN1-resistant long non-coding RNA that affect innate immunity and viral replication. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of XRN1 protein. Immunogen :
Recombinant fusion protein of human XRN1. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
XRN1 monoclonal antibody Background :
5’-3’ exoribonuclease 1 (XRN1) is a cytoplasmic exonuclease that degrades RNA containing a 5’-monophosphate to component mononucleotides. XRN1 is the primary exonuclease associated with ribosomes in the cytoplasm and is responsible for mRNA turnover. This turnover is facilitated in discrete structures in the cytoplasm called P-bodies that contain decapping and deadenylation proteins. XRN1 also plays a role in RISC-mediated mRNA degradation, as it associates with 3’ mRNA fragments generated by RISC cleavage. This process does not require uncapping or deadenylation. XRN1 plays a significant role in viral RNA degradation. As such, many viral genomes, including hepatitis C, Dengue, and West Nile, encode for XRN1-resistant long non-coding RNA that affect innate immunity and viral replication. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of XRN1 protein. Immunogen :
Recombinant fusion protein of human XRN1. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of XRN1 expression in K562 (A), MCF7 (B) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.