LIN28B monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
LIN28B monoclonal antibody Background :
LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs. The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2. It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs. LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy. In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans. Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of LIN28B protein. Immunogen :
Recombinant fusion protein of human LIN28B. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
LIN28B monoclonal antibody Background :
LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs. The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2. It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs. LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy. In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans. Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of LIN28B protein. Immunogen :
Recombinant fusion protein of human LIN28B. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of LIN28B expression in 293 (A), K562 (B), HepG2 (C) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.