PAX8 monoclonal antibody 
Datasheet
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MSDS
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Product Name :
PAX8 monoclonal antibody Background :
Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development. Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity. PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region. PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis; a subset of PAX proteins also maintain functional importance during postnatal development. Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes, with members of subgroups II and III identified as potential mediators of tumor progression. PAX8 is involved in the development of thyroid follicular cells and the expression of thyroid-specific genes. Investigators have associated mutations in the PAX8 gene with thyroid dysgenesis, thyroid follicular carcinomas, and atypical follicular thyroid adenomas. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of PAX8 protein. Immunogen :
Recombinant fusion protein of human PAX8. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
PAX8 monoclonal antibody Background :
Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development. Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity. PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region. PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis; a subset of PAX proteins also maintain functional importance during postnatal development. Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes, with members of subgroups II and III identified as potential mediators of tumor progression. PAX8 is involved in the development of thyroid follicular cells and the expression of thyroid-specific genes. Investigators have associated mutations in the PAX8 gene with thyroid dysgenesis, thyroid follicular carcinomas, and atypical follicular thyroid adenomas. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of PAX8 protein. Immunogen :
Recombinant fusion protein of human PAX8. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of PAX8 expression in HeLa (A), Raji (B), SKOV3 (C) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.