RAB8A monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
RAB8A monoclonal antibody Background :
The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring . Studies show that Rab8 is localized to the trans Golgi network (TGN), basolateral membrane, and vesicular structures where it helps regulate target protein transport between TGN and the basolateral membrane . Overexpression studies and mutation analysis of Rab8 and its associated Rab8GEF indicate additional roles in actin and microtubule remodeling during polarized membrane transport and membrane protrusion formation . Rab8 associates with myosin Vb and is required for translocation of GLUT4 following insulin stimulation in muscle . Control of target protein vesicle transport by Rab8 also regulates MT1-MMP activity during extracellular matrix formation and JRAB/MICAL-L2 at tight junction formation . Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of RAB8A protein. Immunogen :
Recombinant fusion protein of human RAB8A. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
RAB8A monoclonal antibody Background :
The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring . Studies show that Rab8 is localized to the trans Golgi network (TGN), basolateral membrane, and vesicular structures where it helps regulate target protein transport between TGN and the basolateral membrane . Overexpression studies and mutation analysis of Rab8 and its associated Rab8GEF indicate additional roles in actin and microtubule remodeling during polarized membrane transport and membrane protrusion formation . Rab8 associates with myosin Vb and is required for translocation of GLUT4 following insulin stimulation in muscle . Control of target protein vesicle transport by Rab8 also regulates MT1-MMP activity during extracellular matrix formation and JRAB/MICAL-L2 at tight junction formation . Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of RAB8A protein. Immunogen :
Recombinant fusion protein of human RAB8A. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of RAB8A expression in Hela (A), HCT116 (B) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.