Product Name :
RAD23B monoclonal antibody Background :
The yeast nucleotide excision repair (NER) radiation sensitive protein 23 (rad23) and its human homologs Rad23A (hHR23A) and Rad23B (hHR23B) are critical components of the cellular machinery that recognize DNA lesions and serve as receptors that target ubiquitinated substrates to the proteasome for degradation . The UV excision repair protein Rad23B is a multi-domain scaffold protein that plays an important role in ubiquitin-dependent proteasomal degradation. Rad23B contains an amino-terminal ubiquitin-like (UbL) domain that facilitates interaction with the S5a/PSMD4 subunit of the proteasome 19S regulatory complex . In addition, Rad23B contains a central ubiquitin-associated domain (UBA1) and a carboxy-terminal UBA2 domain, which bind mono- and polyubiquitin with distinct specificities . Research studies demonstrate that Rad23B binds specifically to K48-ubiquitinated proteins to facilitate recruitment of target proteins to the proteasome . Between the paired UBA domains, Rad23B contains an XPC-binding domain that facilitates binding to XPC and recruitment to DNA lesions , as well as the binding of peptide:N-glycanase that is critical for recruitment of ubiquitinated ERAD substrates to the proteasome . Research studies have shown that targeted deletion of the murine Rad23b locus impairs embryonic development, suggesting that Rad23B is essential for mammalian development . Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of RAD23B protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human RAD23B. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of RAD23B expression in Jurkat (A), A549 (B), HUVEC (C), NIH3T3 (D), mouse brain (E), rat brain (F) whole cell lysates.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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