RAB27 monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
RAB27 monoclonal antibody Background :
Rab27 is a member of the Ras superfamily of small Rab GTPases implicated in exocytosis . The protein is localized in secretory lysosomes, such as melanosomes in melanocyte or lytic granules in cytotoxic T cells to control exosome secretion pathway. Rab27 has two isoforms, Rab27a and Rab27b. Rab27a colocalizes with part of CD63 staining vesicles, and Rab27b shows perinuclear distribution. Target knock out studies indicate that the isoforms control different steps of the exosome secretion pathway . Rab27a interacts with a wide range of effectors and is involved in multiple steps of exocytosis depending on the effector it associated with and the cell type that is involved . Rab27a has been shown to be an important player in leukocyte function, cancer metastasis and invasion, and insulin secretion. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of RAB27 protein. Immunogen :
Recombinant fusion protein of human RAB27. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
RAB27 monoclonal antibody Background :
Rab27 is a member of the Ras superfamily of small Rab GTPases implicated in exocytosis . The protein is localized in secretory lysosomes, such as melanosomes in melanocyte or lytic granules in cytotoxic T cells to control exosome secretion pathway. Rab27 has two isoforms, Rab27a and Rab27b. Rab27a colocalizes with part of CD63 staining vesicles, and Rab27b shows perinuclear distribution. Target knock out studies indicate that the isoforms control different steps of the exosome secretion pathway . Rab27a interacts with a wide range of effectors and is involved in multiple steps of exocytosis depending on the effector it associated with and the cell type that is involved . Rab27a has been shown to be an important player in leukocyte function, cancer metastasis and invasion, and insulin secretion. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of RAB27 protein. Immunogen :
Recombinant fusion protein of human RAB27. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of RAB27 expression in K562 (A) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.