Product Name :
RCC1 monoclonal antibody Background :
The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport . Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor activity, which catalyzes the exchange of bound GDP for GTP. Regulator of chromatin condensation 1 is the only known RanGEF . RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally. Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation and methylation. RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis . In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclin B/cdc2 . This phosphorylation may play a role in RCC1 interaction with chromatin and RCC1 RanGEF activity . Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of RCC1 protein. Immunogen :
Recombinant fusion protein of human RCC1. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of RCC1 expression in Hela (A), HL60 (B), COS7 (C), C2C12 (D) whole cell lysates.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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