Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
MYOD1 monoclonal antibody MB66892
  • Western blot analysis of MYOD1 expression in A549 (A) whole cell lysates.
Product NameMYOD1 monoclonal antibody
Catalog No.MB66892
Swiss-ProtP15172
Host Mouse
ReactivityHuman
ApplicationsWB
Application_allWB (1/500 - 1/1000)
BiowMW~ 45 kDa
Alternative NameBHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
Purification&Purity
ConjugateUnconjugated
ModificationUnmodification
Browse similar products>>
Size Price
50ul $158
100ul $275
Add to cart My orders
Product Name :
MYOD1 monoclonal antibody
Background :
Myoblast determination protein 1, also called myogenic factor 3 , is a member of the MyoD family of muscle specific bHLH transcription factors . This family is responsible for controlling specification of the muscle cell lineage and members are expressed only in skeletal muscle and its precursors. MyoD1 is considered a master regulator of skeletal myogenesis as its expression can induce myogenic differentiation in myoblasts, fibroblasts, and a variety of other cell types . Through ChIP-sequencing experiments, researchers have discovered that MyoD is associated with the promoters of many genes in muscle cells, but it only regulates a subset of those genes. These research studies point to regulation of MyoD transcriptional activity via epigenetic mechanisms involving SWI/SNF complexes and Polycomb and Trithorax Group proteins . Additional influences on muscle development include signal transduction through MAPK, PI3K/Akt, myostatin, NF-κB, and mTOR signaling pathways.
Product :
Mouse IgM. Supplied in crude ascites with 0.01% sodium azide.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Recognizes endogenous levels of MYOD1 protein.
Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human MYOD1. The exact sequence is proprietary.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of MYOD1 expression in A549 (A) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER