Cytokeratin 18 monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Cytokeratin 18 monoclonal antibody Background :
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and maintain the overall structural integrity of epithelial cells. Cytokeratins are useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin polypeptides are designated 1 to 20 and are divided into the type I and type II subgroups. The type II family members comprise the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. Cytokeratin 5 is expressed in normal basal cells. Mutations of the cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB). Cytokeratin 6 is expressed in suprabasal keratinocytes of wounded epidermis and in acrosyringeal keratinocytes in normal skin. Cytokeratin 18 is one of the first keratins expressed in the embryo, and persists into adult tissues. It is a major component of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas. Product :
Mouse IgG3. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of Cytokeratin 18 protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 18. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
Cytokeratin 18 monoclonal antibody Background :
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and maintain the overall structural integrity of epithelial cells. Cytokeratins are useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin polypeptides are designated 1 to 20 and are divided into the type I and type II subgroups. The type II family members comprise the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. Cytokeratin 5 is expressed in normal basal cells. Mutations of the cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB). Cytokeratin 6 is expressed in suprabasal keratinocytes of wounded epidermis and in acrosyringeal keratinocytes in normal skin. Cytokeratin 18 is one of the first keratins expressed in the embryo, and persists into adult tissues. It is a major component of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas. Product :
Mouse IgG3. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of Cytokeratin 18 protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 18. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of Cytokeratin 18 expression in HepG2 (A), K562 (B), NCIH460 (C) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.