ISG15 monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
ISG15 monoclonal antibody Background :
Interferon-induced 15 kDa protein (ISG15) acts as ubiquitin by conjugating to intracellular target proteins such as JAK1 or MAPK3/ERK1 through an enzyme pathway distinct from that of ubiquitin. ISG15 shows specific chemotactic activity towards neutrophils and activates them to induce the release of eosinophil chemotactic factors. ISG15 is also involved in paracrine, autocrine and endocrine mechanisms by inducing IFN-g secretion by monocytes and macrophages, as in cell-to-cell signaling. ISG15 is a cytoplasmic protein expressed mainly in muscle, epithelial, neuronal and lymphoid cells. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of ISG15 protein. Immunogen :
Recombinant fusion protein of human ISG15. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
ISG15 monoclonal antibody Background :
Interferon-induced 15 kDa protein (ISG15) acts as ubiquitin by conjugating to intracellular target proteins such as JAK1 or MAPK3/ERK1 through an enzyme pathway distinct from that of ubiquitin. ISG15 shows specific chemotactic activity towards neutrophils and activates them to induce the release of eosinophil chemotactic factors. ISG15 is also involved in paracrine, autocrine and endocrine mechanisms by inducing IFN-g secretion by monocytes and macrophages, as in cell-to-cell signaling. ISG15 is a cytoplasmic protein expressed mainly in muscle, epithelial, neuronal and lymphoid cells. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of ISG15 protein. Immunogen :
Recombinant fusion protein of human ISG15. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of ISG15 expression in Hela (A), MCF7 (B) whole cell lysates. -
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.