Product Name :
Vitronectin monoclonal antibody Background :
Fibronectin and Vitronectin are extracellular matrix glycoproteins that are present on most cell surfaces, in extracellular fluids, and in plasma. Both Fibronectin and Vitronectin have been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Vitronectin contains an RGD (Arg-Gly-Asp acid) sequence that is present in many cell adhesion ligands. The RGD sequence has been shown to be essential for cell adhesion. Increased expression of Vitronectin, integrins and plasminogen activators has been observed in migrating cells during wound healing. Vitronectin has been shown to enhance smooth cell migration, and PAI-1 has been shown to bind to Vitronectin with high affinity, resulting in the blocking of smooth cell migration. Glycosaminoglycans, proteins involved in the anchoring of Vitronectin to the extracellular matrix, have been shown to stimulate the cleavage of Vitronectin by plasmin. This cleavage reduces the affinity of Vitronectin for PAI-1. Product :
Mouse IgM. Supplied in crude ascites with 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of Vitronectin protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Vitronectin. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of Vitronectin expression in T47D (A) whole cell lysates.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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