Product Name :
PGK1 monoclonal antibody Background :
Phosphoglycerate kinases 1/2 (PGK1/2, ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) are somatically expressed, glycolytic enzymes that catalyze the transfer of a phosphoryl group from the acyl phosphate of 1,3-bisphosphoglycerate to ADP, thereby forming ATP and 3-phosphoglycerate. The human PGK gene is interrupted by 10 introns and spans 23 kilobases, and is X chromosome-linked at position Xq21.1, a region implicated in prostate cancer, androgen insensitivity, perineal hypospadias, and other genetic abnormalities. In addition to influencing glycolysis, the PGK1 is secreted by tumor cells and contributes to proliferative angiogenic processes as a disulfide reductase. PGK1 mediated reduction of disulphide bonds in the serine proteinase plasmin initiates the release of the tumor blood vessel inhibitor angiostatin, an event that is critical for blood vessel formation or angiogenesis in tumor expansion and metastasis. Product :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of PGK1 protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of PGK1 expression in HEK293T (A), A431 (B), mouse brain (C) whole cell lysates.
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  Immunohistochemical analysis of PGK1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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