USP2 monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
USP2 monoclonal antibody Background :
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove Ub, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP2 (Ub specific peptidase 2), also known as 41 kDa Ub-specific protease, is a 605 amino acid deubiquitinating enzyme that participates in the Ub pathway. Localized to the cytoplasm, USP2 forms a homooligomer and catalyzes the reaction of the Ub C-terminal thioester with water to form Ub and a thiol. USP2 is expressed as two isoforms produced by alternative splicing. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of USP2 protein. Immunogen :
Recombinant fusion protein of human USP2. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
USP2 monoclonal antibody Background :
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove Ub, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP2 (Ub specific peptidase 2), also known as 41 kDa Ub-specific protease, is a 605 amino acid deubiquitinating enzyme that participates in the Ub pathway. Localized to the cytoplasm, USP2 forms a homooligomer and catalyzes the reaction of the Ub C-terminal thioester with water to form Ub and a thiol. USP2 is expressed as two isoforms produced by alternative splicing. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of USP2 protein. Immunogen :
Recombinant fusion protein of human USP2. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of USP2 expression in MCF7 (A), PC3 (B), COS7 (C), Du145 (D), SKBR3 (E) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.