Product Name :
CBFA2T3 monoclonal antibody Background :
ETO and ETO-2, which are alternatively designated MTG8 and MTG16, respectively, are members of the ETO transcription factor family. These transcription factors are characterized by a zinc-finger domain and four conserved domains, of which domain II is required for dimerization between family members. ETO and ETO-2 may function to mediate interactions between DNA binding proteins and transcriptional regulators, such as N-CoR. Frequently, the t(8;21) translocation of ETO produces the AML-1/ETO oncoprotein, which consists of the first 177 amino acids of AML-1 and all but the first 30 amino acids of ETO. AML-1/ETO expression is observed in 12-15% of acute myelogenous, M2 subtype leukemias. The AML-1/ETO fusion proteins associate with multimeric N-CoR/mSin3/HDAC1 complexes, block differentiation and induce transcriptional repression by altering chromatin remodeling. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of CBFA2T3 protein. Immunogen :
Recombinant fusion protein of human CBFA2T3. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of CBFA2T3 expression in Jurkat (A), CCRFCEM (B) whole cell lysates.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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