TIP47 monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
TIP47 monoclonal antibody Background :
Tail-interacting 47kDa protein (TIP47), known also as human placental tissue protein 17b (PP17b), binds to cytoplasmic domains of the cation-dependent (CD) and cation-independent (CI) mannose 6-phosphate receptors (MPRs) and facilitates their transport from endosomes to the Golgi complex. The inability of TIP47 to bind several proteins also transported from endosomes to the trans Golgi network indicates that TIP47 associates with a very select set of cargo molecules. In CD-MPR, TIP47 recognizes a phenylalanine/tryptophan signal sequence essential for proper sorting within the endosomal pathway.For CI-MPR binding, TIP47 requires cytoplasmic residues 48-74 of CI-MPR for high-affinity binding while residues 75-163 of CI-MPR aid in the presentation of the higher-affinity residues. Additionally, TIP47 competes with AP-2 clathrin adaptor for binding residues 24-29 of CI-MPR. In tissue extracts of cervical carcinoma patients, TIP47 is overexpressed. Dysplastic cells in high grade dysplasias express more TIP47 than dysplastic cells in low grade dysplasias, and both cytoplasmic types of dysplasias express more TIP47 than normal cervical epithelial cells. The gene encoding human TIP47 maps to chromosome 19p13.3. Product :
Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of TIP47 protein. Immunogen :
KLH-conjugated synthetic peptide of human TIP47. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
TIP47 monoclonal antibody Background :
Tail-interacting 47kDa protein (TIP47), known also as human placental tissue protein 17b (PP17b), binds to cytoplasmic domains of the cation-dependent (CD) and cation-independent (CI) mannose 6-phosphate receptors (MPRs) and facilitates their transport from endosomes to the Golgi complex. The inability of TIP47 to bind several proteins also transported from endosomes to the trans Golgi network indicates that TIP47 associates with a very select set of cargo molecules. In CD-MPR, TIP47 recognizes a phenylalanine/tryptophan signal sequence essential for proper sorting within the endosomal pathway.For CI-MPR binding, TIP47 requires cytoplasmic residues 48-74 of CI-MPR for high-affinity binding while residues 75-163 of CI-MPR aid in the presentation of the higher-affinity residues. Additionally, TIP47 competes with AP-2 clathrin adaptor for binding residues 24-29 of CI-MPR. In tissue extracts of cervical carcinoma patients, TIP47 is overexpressed. Dysplastic cells in high grade dysplasias express more TIP47 than dysplastic cells in low grade dysplasias, and both cytoplasmic types of dysplasias express more TIP47 than normal cervical epithelial cells. The gene encoding human TIP47 maps to chromosome 19p13.3. Product :
Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of TIP47 protein. Immunogen :
KLH-conjugated synthetic peptide of human TIP47. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of TIP47 expression in Hela (A), K562 (B), Daudi (C) whole cell lysates. -
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.