RNF81 monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
RNF81 monoclonal antibody Background :
Tripartite motif-containing protein 21 (TRIM21) is a cytosolic antiviral protein that is induced by interferons and acts as a cytosolic IgG receptor. TRIM21 is an activator of the innate immune system and targets antibody bound virions to the proteasome via its E3 ubiquitin ligase activity, leading to degradation of the virions in the cytosol. Interestingly, TRIM21 has also been shown to negatively regulate pathogen-induced interferon production through the polyubiquitin-mediated degradation of multiple targets including IRF3, IRF7, and DDX41. Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of RNF81 protein. Immunogen :
Purified recombinant human TRIM21 protein fragments expressed in E.coli. Conjugate :
Unconjugated Modification :
Unmodification
RNF81 monoclonal antibody Background :
Tripartite motif-containing protein 21 (TRIM21) is a cytosolic antiviral protein that is induced by interferons and acts as a cytosolic IgG receptor. TRIM21 is an activator of the innate immune system and targets antibody bound virions to the proteasome via its E3 ubiquitin ligase activity, leading to degradation of the virions in the cytosol. Interestingly, TRIM21 has also been shown to negatively regulate pathogen-induced interferon production through the polyubiquitin-mediated degradation of multiple targets including IRF3, IRF7, and DDX41. Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of RNF81 protein. Immunogen :
Purified recombinant human TRIM21 protein fragments expressed in E.coli. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of RNF81 expression in MCF7 (A), 293T (B), Hela (C) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.