WNT3A monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
WNT3A monoclonal antibody Background :
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity).Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).A palmitoylation site was proposed at Cys-77, but it was later shown that this cysteine is engaged in a disulfide bond. Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of WNT3A protein. Immunogen :
Purified recombinant Wnt3a protein fragments expressed in E.coli. Conjugate :
Unconjugated Modification :
Unmodification
WNT3A monoclonal antibody Background :
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity).Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).A palmitoylation site was proposed at Cys-77, but it was later shown that this cysteine is engaged in a disulfide bond. Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of WNT3A protein. Immunogen :
Purified recombinant Wnt3a protein fragments expressed in E.coli. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot analysis of WNT3A expression in MCF7 (A), ZR751 (B), MDAMB468 (C), T47D (D) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.