ACLY monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
ACLY monoclonal antibody Background :
ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol and acetylcholine, as well as for glucogenesis . Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase . It is phosphorylated by GSK-3 on Thr446 and Ser450 . Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt . Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme . Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of ACLY protein. Immunogen :
Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli. Conjugate :
Unconjugated Modification :
Unmodification
ACLY monoclonal antibody Background :
ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol and acetylcholine, as well as for glucogenesis . Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase . It is phosphorylated by GSK-3 on Thr446 and Ser450 . Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt . Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme . Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of ACLY protein. Immunogen :
Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of ACLY expression in NIH3T3 (A), K562 (B), COS7 (C), Hela (D) whole cell lysates. -
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.