Product Name :
IMPDH2 Rabbit monoclonal antibody Background :
Inosine-5'-monophosphate dehydrogenase (IMPDH) catalyzes the conversion of IMP to XMP, the first and rate-limiting step of guanine nucleotide biosynthesis. The isozymes IMPDH1 and IMPDH2 are encoded by two distinct genes. Research studies show that IMPDH2 is overexpressed in glioblastoma. In glioblastoma, IMPDH2-mediated GTP biosynthesis is essential for rRNA and tRNA synthesis and the formation of enlarged and irregular nucleoli. In addition, IMPDH1 and IMPDH2 double knockout leads to significant inhibition of tumor growth in an orthotopic glioblastoma model. Furthermore, IMPDH1 and IMPDH2 expression is upregulated in small cell lung cancer cells with low expression of ASCL1 transcription factor (ASCL1Low). Suppression of IMPDH activity inhibits ASCL1Low small cell lung cancer growth. Product :
Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of IMPDH2 protein. Immunogen :
A synthetic peptide of human IMPDH2 Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of IMPDH2 expression in NIH3T3 (A), mouse liver (B), Hela (C), A549 (D), HL60 (E), U2OS (F), C6 (G) whole cell lysates.
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  Immunohistochemical analysis of IMPDH2 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.21). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunohistochemical analysis of IMPDH2 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.21). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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