ABCE1 Rabbit monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
ABCE1 Rabbit monoclonal antibody Background :
ABCE1 (RNS4I, RLI) is a mediator of the 2-5A/RNase L pathway. The 2-5A/RNase L system is considered an essential pathway of interferon (IFN) action. In the pathway, IFN stimulation activates 2-5A synthetases which convert ATP into a set of atypical oligomers known as 2-5A. These oligomers in turn activate RNase L (RNS4), which leads to inhibition of protein synthesis by cleaving mRNAs at the 3-prime side of UpNp sequences. This inhibition necessitates the association of ABCE1 with RNase L and is dependent on the ratio between the two proteins. The 2-5A/RNase L system could also play a more general physiological role, for instance, in the regulation of RNA stability in mammalian cells. Product :
Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of ABCE1 protein. Immunogen :
A synthetic peptide of human ABCE1 Conjugate :
Unconjugated Modification :
Unmodification
ABCE1 Rabbit monoclonal antibody Background :
ABCE1 (RNS4I, RLI) is a mediator of the 2-5A/RNase L pathway. The 2-5A/RNase L system is considered an essential pathway of interferon (IFN) action. In the pathway, IFN stimulation activates 2-5A synthetases which convert ATP into a set of atypical oligomers known as 2-5A. These oligomers in turn activate RNase L (RNS4), which leads to inhibition of protein synthesis by cleaving mRNAs at the 3-prime side of UpNp sequences. This inhibition necessitates the association of ABCE1 with RNase L and is dependent on the ratio between the two proteins. The 2-5A/RNase L system could also play a more general physiological role, for instance, in the regulation of RNA stability in mammalian cells. Product :
Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of ABCE1 protein. Immunogen :
A synthetic peptide of human ABCE1 Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of ABCE1 expression in K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.