DTX3L polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
DTX3L polyclonal antibody Background :
The E3 ubiquitin-protein ligase DTX3L (BBAP) is a deltex (DTX) family ligase that binds B aggressive lymphoma 1 (BAL1) to mediate the monoubiquitination of histone H4 Lys91 in response to DNA damage. The early recruitment of poly-ADP-ribose polymerase 1 (PARP1) protein to sites of DNA damage leads to poly-ADP-ribosylation (PARylation) of multiple target proteins. This results in the subsequent recruitment of DTX3L/BAL1 through binding of the BAL macrodomain to PARylated proteins. DTX3L/BAL1 then mediates the monoubiquitination of histone H4 Lys91 that is required for subsequent methylation of histone H4 Lys20 and recruitment of p53-binding protein 1. DTX3L and BAL1 are highly expressed in malignant B-cell lymphomas and act to confer chemotherapy-resistance by protecting cells from DNA damaging agents. Product :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of DTX3L protein. Immunogen :
Recombinant protein corresponding to human DTX3L. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
DTX3L polyclonal antibody Background :
The E3 ubiquitin-protein ligase DTX3L (BBAP) is a deltex (DTX) family ligase that binds B aggressive lymphoma 1 (BAL1) to mediate the monoubiquitination of histone H4 Lys91 in response to DNA damage. The early recruitment of poly-ADP-ribose polymerase 1 (PARP1) protein to sites of DNA damage leads to poly-ADP-ribosylation (PARylation) of multiple target proteins. This results in the subsequent recruitment of DTX3L/BAL1 through binding of the BAL macrodomain to PARylated proteins. DTX3L/BAL1 then mediates the monoubiquitination of histone H4 Lys91 that is required for subsequent methylation of histone H4 Lys20 and recruitment of p53-binding protein 1. DTX3L and BAL1 are highly expressed in malignant B-cell lymphomas and act to confer chemotherapy-resistance by protecting cells from DNA damaging agents. Product :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of DTX3L protein. Immunogen :
Recombinant protein corresponding to human DTX3L. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of DTX3L expression in mouse thymus (A) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.