Product Name :
Adenylate Kinase 1 polyclonal antibody Background :
Adenylate kinases 1-5 (designated AK1-5) are a set of enzymes that regulate the phosphorylation state of intracellular adenine nucleotides, which are the principle high-energy phosphoryl-carrying molecules in living cells. AKs influence metabolic signals, which include gene expression, ion channel activity and protein kinase-mediated signaling, by catalyzing phosphoryl transfer between adenine nucleotides (AMP, ADP, ATP). Inherited mutations leading to AK deficiencies in erythrocytes have been implicated in hemolytic anemia. Human AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes and is clustered within myofibrils or bound to membranes. AK1-mediated phosphotransfer is essential for maintaining sufficient cellular energy, which enables proper skeletal muscle performance and metabolic activity. Product :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of Adenylate Kinase 1 protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Adenylate Kinase 1. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of Adenylate Kinase 1 expression in A549 (A), A375 (B) whole cell lysates.
• 
  Immunohistochemical analysis of Adenylate Kinase 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of Adenylate Kinase 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---