Product Name :
PAR1 polyclonal antibody Background :
PAR1 (F2R) belongs to the PAR (Protease-Activated Receptor) family of G protein-coupled receptors. These membrane receptors are activated through amino terminal cleavage of the receptor by serine proteases such as thrombin, trypsin, or matrix metalloproteinases . Protease-mediated cleavage exposes the ‘tethered-ligand’ fragment of the receptor; this binds to a second extracellular loop of the receptor and triggers receptor activation. Activated PAR1 couples to distinct G-protein complexes or arrestins, further relaying signals to downstream players (e.g., phospholipase C, Rho-kinase) . PAR1 function is necessary for normal physiological processes such as hemostasis, platelet activation, and thrombosis, whereas abnormal activation of PAR1 has been associated with inflammatory disorders, cardiovascular diseases, and metastasis. Product :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of PAR1 protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PAR1. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of PAR1 expression in U2OS (A) whole cell lysates.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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