PLA2G2D polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
PLA2G2D polyclonal antibody Background :
Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycerophospholipid molecules. These enzymes are generally calcium-dependent and have been found both intra- and extracellularly. By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids. One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids, many of which are potent mediators of inflammation. As a member of the PLA2 family, group IID sPLA2, also known as PLA2G2D (phosphatidylcholine 2-acylhydrolase 2D), sPLA2-IID or SPLASH, is a 145 amino acid secreted protein that is broadly expressed but found at highest levels in pancreas and spleen. Group IID sPLA2 catalyzes the reaction of phosphatidylcholine and water into 1-acylglycerophosphocholine and carboxylate, and has been liked to body weight loss in patients with chronic obstructive pulmonary disease (COPD), as well as lymphotoxin deficiency. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PLA2G2D polyclonal antibody detects endogenous levels of PLA2G2D protein. Immunogen :
Synthetic peptide, corresponding to Human PLA2G2D. Conjugate :
Unconjugated Modification :
Unmodification
PLA2G2D polyclonal antibody Background :
Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycerophospholipid molecules. These enzymes are generally calcium-dependent and have been found both intra- and extracellularly. By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids. One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids, many of which are potent mediators of inflammation. As a member of the PLA2 family, group IID sPLA2, also known as PLA2G2D (phosphatidylcholine 2-acylhydrolase 2D), sPLA2-IID or SPLASH, is a 145 amino acid secreted protein that is broadly expressed but found at highest levels in pancreas and spleen. Group IID sPLA2 catalyzes the reaction of phosphatidylcholine and water into 1-acylglycerophosphocholine and carboxylate, and has been liked to body weight loss in patients with chronic obstructive pulmonary disease (COPD), as well as lymphotoxin deficiency. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PLA2G2D polyclonal antibody detects endogenous levels of PLA2G2D protein. Immunogen :
Synthetic peptide, corresponding to Human PLA2G2D. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of sPLA2-IID expression in Hela (A), THP1 (B) whole cell lysates. -
Immunofluorescent analysis of sPLA2-IID staining in U2OS cells.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.