Product Name :
TOMM20 polyclonal antibody Background :
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TOMM20 polyclonal antibody detects endogenous levels of TOMM20 protein. Immunogen :
Recombinant fusion protein containing a sequence corresponding to amino acids 1-397 of human SETDB1 (NP_001230420.1). Conjugate :
Unconjugated Modification :
Unmodification

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  Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug TOMM20 antibody. Western blot was performed from the immunoprecipitate using TOMM20 antibody at a dilution of 1:1000.
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  Immunohistochemistry of paraffin-embedded Mouse kidney using TOMM20 pAb at dilution of 1:100 (40x lens).
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  Immunohistochemistry of paraffin-embedded Mouse kidney using TOMM20 pAb at dilution of 1:100 (40x lens).
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  Immunohistochemistry of paraffin-embedded Mouse kidney using TOMM20 pAb at dilution of 1:100 (40x lens).

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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