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AKT polyclonal antibody AP0059
  • Western blot (WB) analysis of Akt polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of AKT pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
  • Immunohistochemistry (IHC) analyzes of AKT pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
  • Immunohistochemistry (IHC) analyzes of AKT pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Product NameAKT polyclonal antibody
Catalog No.AP0059
Swiss-ProtP31749/P31751/Q9Y243
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF IP
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200
BiowMW~ 60 kDa
Alternative NameRAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; PKBβ; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; AKT3; PKBG; PKBγ
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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100ul $275
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Product Name :
AKT polyclonal antibody
Background :
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
AKT polyclonal antibody detects endogenous levels of total AKT protein.
Immunogen :
Recombinant full length Human AKT1.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Akt polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of AKT pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
  • Immunohistochemistry (IHC) analyzes of AKT pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
  • Immunohistochemistry (IHC) analyzes of AKT pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Epigenetic down regulation of G protein-coupled estrogen receptor (GPER) functions as a tumor suppressor in colorectal cancer PMCID:    Pubmed No.:28476123 Madecassic acid, the contributor to the anti-colitis effect of madecassoside, enhances the shift of Th17 toward Treg cells via the PPARγ/AMPK/ACC1 pathway PMCID:    Pubmed No.:28358365 The tumor-suppressor gene LZTS1 suppresses colorectal cancer proliferation through inhibition of the AKT–mTOR signaling pathway PMCID:    Pubmed No.:25667121 TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways PMCID:    Pubmed No.:27669982 TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways PMCID:    Pubmed No.:27669982 Bamboo leaf extract ameliorates diabetic nephropathy through activating the AKT signaling pathway in rats PMCID:    Pubmed No.:28359892 Activation of GPER suppresses epithelial mesenchymal transition of triple negative breast cancer cells via NF-κB signals PMCID:    Pubmed No.:26842883 Proton pump inhibitor pantoprazole abrogates adriamycin-resistant gastric cancer cell invasiveness via suppression of Akt/GSK-β/β-catenin signaling and epithelial–mesenchymal transition PMCID:    Pubmed No.:25449432 White light emitting diode suppresses proliferation and induces apoptosis in hippocampal neuron cells through mitochondrial cytochrome c oxydase-mediated IGF-1 and TNF-α pathways PMCID:    Pubmed No.:29106990 Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells PMCID:    Pubmed No.:25857968 miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/AKT pathway in nasopharyngeal carcinoma PMCID:    Pubmed No.:27010857 Cellular proliferation and differentiation induced by single-layer molybdenum disulfide and mediation mechanisms of proteins via the Akt-mTOR-p70S6K signaling pathway PMCID:    Pubmed No.:28714804 Cucurmosin induces apoptosis of BxPC-3 human pancreatic cancer cells via inactivation of the EGFR signaling pathway. PMCID:    Pubmed No.:22139427 Dose-dependent effect of tamoxifen in tamoxifen-resistant breast cancer cells via stimulation by the ERK1/2 and AKT signaling pathways. PMCID:    Pubmed No.:23338782 Activation of cannabinoid type 2 receptor by JWH133 protects heart against ischemia/reperfusion-induced apoptosis. PMCID:    Pubmed No.:23711495 MicroRNA-34a Suppresses Cell Proliferation by Targeting LMTK3 in Human Breast Cancer MCF-7 Cell Line PMCID:    Pubmed No.:24050776 MicroRNA-30b functions as a tumour suppressor in human colorectal cancer by targeting KRAS, PIK3CD and BCL2 PMCID:    Pubmed No.:24293274 ART1 Silencing Enhances Apoptosis of Mouse CT26 Cells via the PI3K/Akt/NF-κB Pathway PMCID:    Pubmed No.:24335275 Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway PMCID:    Pubmed No.:24366110 LW-214, a newly synthesized flavonoid, induces intrinsic apoptosis pathway by down-regulating Trx-1 in MCF-7 human breast cells PMCID:    Pubmed No.:24374359 cyclooxygenase-2 inhibitor is a robust enhancer of anticancer agents against hepatocellular carcinoma multicellular spheroids PMCID:    Pubmed No.:24591842 Ribonuclease inhibitor up-regulation inhibits the growth and induces apoptosis in murine melanoma cells through repression of angiogenin and ILK/PI3K/AKT signaling pathway. PMCID:    Pubmed No.:24769129 High uric acid directly inhibits insulin signalling and induces insulin resistance PMCID:    Pubmed No.:24769205 GPER mediates enhanced cell viability and motility via non-genomic signaling induced by 17β-estradiol in triple-negative breast cancer cells PMCID:    Pubmed No.:24874276 The differential susceptibilities of MCF-7 and MDA-MB-231 cells to the cytotoxic effects of curcumin are associated with the PI3K/Akt-SKP2-Cip/Kips pathway PMCID:    Pubmed No.:25530715 A novel Pseudolaric acid B derivative, Hexahydropseudolaric acid B, exterts immunomodulatory effect in vitro/in vivo evaluation PMCID:    Pubmed No.:25446920 Neuroprotection by Kukoamine A against oxidative stress may involve N-methyl-D-aspartate receptors PMCID:    Pubmed No.:25445711 Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells PMCID:    Pubmed No.:25193113 Dietary betaine supplementation to gestational sows enhances hippocampal IGF2 expression in newborn piglets with modified DNA methylation of the differentially methylated regions. PMCID:    Pubmed No.:25410747 Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway PMCID:    Pubmed No.:25564356 Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells PMCID:    Pubmed No.:25857968 Frequent amplification of PTP1B is associated with poor survival of gastric cancer patients PMCID:    Pubmed No.:25590580 PM2. 5‐induced oxidative stress increases adhesion molecules expression in human endothelial cells through the ERK/AKT/NF‐κB‐dependent pathway PMCID:    Pubmed No.:25876056 Arctigenin exerts anti-colitis efficacy through inhibiting the differentiation of Th1 and Th17 cells via an mTORC1-dependent pathway PMCID:    Pubmed No.:26074264 Ethanolic Ginkgo biloba leaf extract prevents renal fibrosis through Akt/mTOR signaling in diabetic nephropathy PMCID:    Pubmed No.:26547529 Neuroglobin Attenuates Beta Amyloid-Induced Apoptosis Through Inhibiting Caspases Activity by Activating PI3K/Akt Signaling Pathway PMCID:    Pubmed No.:26346601 Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo PMCID:    Pubmed No.:26531674 Exogenous avian leukosis virus-induced activation of the ERK/AP1 pathway is required for virus replication and correlates with virus-induced tumorigenesis PMCID:    Pubmed No.:26754177 Activation of GPER suppresses epithelial mesenchymal transition of triple negative breast cancer cells via NF-κB signals PMCID:    Pubmed No.:26160845 Heat shock protein 27 downstream of P38-PI3K/Akt signaling antagonizes melatonin-induced apoptosis of SGC-7901 gastric cancer cells PMCID:    Pubmed No.:26877709 Facilitatory effects of fetuin-A on atherosclerosis PMCID:    Pubmed No.:26828753 High Uric Acid Induces Insulin Resistance in Cardiomyocytes In Vitro and In Vivo PMCID:    Pubmed No.:26836389 miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/ AKT pathway in nasopharyngeal carcinoma PMCID:    Pubmed No.:27010857 The differential susceptibilities of MCF-7 and MDA-MB-231 cells to the cytotoxic effects of curcumin are associated with the PI3K/Akt-SKP2-Cip/Kips pathway PMCID:    Pubmed No.:25530715 A novel Pseudolaric acid B derivative, Hexahydropseudolaric acid B, exterts immunomodulatory effect in vitro/in vivo evaluation PMCID:    Pubmed No.:25446920 Neuroprotection by Kukoamine A against oxidative stress may involve N-methyl-D-aspartate receptors PMCID:    Pubmed No.:25445711 Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells PMCID:    Pubmed No.:25193113 Dietary betaine supplementation to gestational sows enhances hippocampal IGF2 expression in newborn piglets with modified DNA methylation of the differentially methylated regions. PMCID:    Pubmed No.:25410747 Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway PMCID:    Pubmed No.:25564356 Ethanolic Ginkgo biloba leaf extract prevents renal fibrosis through Akt/mTOR signaling in diabetic nephropathy PMCID:    Pubmed No.:26547529 Neuroglobin Attenuates Beta Amyloid-Induced Apoptosis Through Inhibiting Caspases Activity by Activating PI3K/Akt Signaling Pathway PMCID:    Pubmed No.:26346601 Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo PMCID:    Pubmed No.:26531674 Exogenous avian leukosis virus-induced activation of the ERK/AP1 pathway is required for virus replication and correlates with virus-induced tumorigenesis PMCID:    Pubmed No.:26754177 Activation of GPER suppresses epithelial mesenchymal transition of triple negative breast cancer cells via NF-κB signals PMCID:    Pubmed No.:26160845 Heat shock protein 27 downstream of P38-PI3K/Akt signaling antagonizes melatonin-induced apoptosis of SGC-7901 gastric cancer cells PMCID:    Pubmed No.:26877709 Facilitatory effects of fetuin-A on atherosclerosis PMCID:    Pubmed No.:26828753 High Uric Acid Induces Insulin Resistance in Cardiomyocytes In Vitro and In Vivo PMCID:    Pubmed No.:26836389 Ribonuclease inhibitor up-regulation inhibits the growth and induces apoptosis in murine melanoma cells through repression of angiogenin and ILK/PI3K/AKT signaling pathway. PMCID:    Pubmed No.:24769129 High uric acid directly inhibits insulin signalling and induces insulin resistance PMCID:    Pubmed No.:24769205 MicroRNA-30b functions as a tumour suppressor in human colorectal cancer by targeting KRAS, PIK3CD and BCL2 PMCID:    Pubmed No.:24293274 ART1 Silencing Enhances Apoptosis of Mouse CT26 Cells via the PI3K/Akt/NF-κB Pathway PMCID:    Pubmed No.:24335275 Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway PMCID:    Pubmed No.:24366110 LW-214, a newly synthesized flavonoid, induces intrinsic apoptosis pathway by down-regulating Trx-1 in MCF-7 human breast cells PMCID:    Pubmed No.:24374359 cyclooxygenase-2 inhibitor is a robust enhancer of anticancer agents against hepatocellular carcinoma multicellular spheroids PMCID:    Pubmed No.:24591842 Heat shock protein 27 downstream of P38-PI3K/Akt signaling antagonizes melatonin-induced apoptosis of SGC-7901 gastric cancer cells PMCID:    Pubmed No.:26877709 Interplay between intergrin-linked kinase and ribonuclease inhibitor affects growth and metastasis of bladder cancer through signaling ILK pathways PMCID:    Pubmed No.:27576342 Korean Red Ginseng improves atopic dermatitis-like skin lesions by suppressing expression of proinflammatory cytokines and chemokines in vivo and in vitro PMCID:    Pubmed No.:28413317 Therapeutic inhibition of phospholipase D1 suppresses hepatocellular carcinoma PMCID:    Pubmed No.:27129182 Persistent Transmissible Gastroenteritis Virus Infection Enhances Enterotoxigenic Escherichia coli K88 Adhesion by Promoting Epithelial-Mesenchymal Transition in Intestinal Epithelial Cells | Journal of Virology PMCID:    Pubmed No.:28794036 Arctigenin exerts anti-colitis efficacy through inhibiting the differentiation of Th1 and Th17 cells via an mTORC1-dependent pathway PMCID:    Pubmed No.:26074264 β-d-glucan from Antrodia Camphorata ameliorates LPS-induced inflammation and ROS production in human hepatocytes PMCID:    Pubmed No.:28642114 Activation of mTOR signaling mediates the increased expression of AChE in high glucose condition: in vitro and in vivo evidences PMCID:    Pubmed No.:26374551 Ethanolic Ginkgo biloba leaf extract prevents renal fibrosis through Akt/mTOR signaling in diabetic nephropathy PMCID:    Pubmed No.:26547529 The differential susceptibilities of MCF-7 and MDA-MB-231 cells to the cytotoxic effects of curcumin are associated with the PI3K/Akt-SKP2-Cip/Kips pathway PMCID:    Pubmed No.:25530715 Alpinetin inhibits lung cancer progression and elevates sensitization drug-resistant lung cancer cells to cis-diammined dichloridoplatium PMCID:    Pubmed No.:26604699 Metformin ameliorates high uric acid-induced insulin resistance in skeletal muscle cells PMCID:    Pubmed No.:28042024 Zeaxanthin improves diabetes-induced cognitive deficit in rats through activiting PI3K/AKT signaling pathway PMCID:    Pubmed No.:28599877 A variation in KCNQ1 gene is associated with repaglinide efficacy on insulin resistance in Chinese Type 2 Diabetes Mellitus Patients PMCID:    Pubmed No.:27857189 Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats PMCID:    Pubmed No.:28300221 IL-6 Inhibits Starvation-induced Autophagy via the STAT3/Bcl-2 Signaling Pathway PMCID:    Pubmed No.:26549519 Korean Byungkyul - Citrus platymamma Hort.et Tanaka flavonoids induces cell cycle arrest and apoptosis, regulating MMP protein expression in Hep3B hepatocellular carcinoma cells PMCID:    Pubmed No.:28035361 Quercetin inhibited epithelial mesenchymal transition in diabetic rats, high-glucose-cultured lens, and SRA01/04 cells through transforming growth factor-β2/phosphoinositide 3-kinase/Akt pathway PMCID:    Pubmed No.:28501572 Dioscin, a potent ITGA5 inhibitor, reduces the synthesis of collagen against liver fibrosis: Insights from SILAC-based proteomics analysis PMCID:    Pubmed No.:28689917 Paeoniflorin suppresses lipid accumulation and alleviates insulin resistance by regulating the Rho kinase/IRS-1 pathway in palmitate-induced HepG2Cells PMCID:    Pubmed No.:28380411 Frequent amplification of PTP1B is associated with poor survival of gastric cancer patients PMCID:    Pubmed No.:25590580 PM2.5-induced oxidative stress increases adhesion molecules expression in human endothelial cells through the ERK/AKT/NF-κB-dependent pathway PMCID:    Pubmed No.:25876056 Neuroprotection by Kukoamine A against oxidative stress may involve N-methyl-d-aspartate receptors PMCID:    Pubmed No.:25445711 Neuroprotective Effects of Clostridium butyricum against Vascular Dementia in Mice via Metabolic Butyrate PMCID:    Pubmed No.:26523278 Neuroprotective Effect of Sodium Butyrate against Cerebral Ischemia/Reperfusion Injury in Mice PMCID:    Pubmed No.:26064905 A novel Pseudolaric acid B derivative, Hexahydropseudolaric acid B, exterts an immunomodulatory effect in vitro/in vivo evaluation PMCID:    Pubmed No.:25446920 Clostridium butyricum attenuates cerebral ischemia/reperfusion injury in diabetic mice via modulation of gut microbiota PMCID:    Pubmed No.:27037183 Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway PMCID:    Pubmed No.:27592453 Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo PMCID:    Pubmed No.:26531674 Tris(2‐chloroethyl)phosphate‐induced cell growth arrest via attenuation of SIRT1‐independent PI3K/Akt/mTOR pathway PMCID:    Pubmed No.:26378621 ONTD induces growth arrest and apoptosis of human hepatoma Bel-7402 cells though a peroxisome proliferator-activated receptor γ-dependent pathway PMCID:    Pubmed No.:28834734 Knockdown of Astrocyte Elevated Gene-1 Inhibits Activation of Hepatic Stellate Cells PMCID:    Pubmed No.:26879903 G protein-coupled estrogen receptor (GPER) mediates NSCLC progression induced by 17β-estradiol (E 2 ) and selective agonist G1 PMCID:    Pubmed No.:25744245 Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway PMCID:    Pubmed No.:25564356 Neuroglobin Attenuates Beta Amyloid-Induced Apoptosis Through Inhibiting Caspases Activity by Activating PI3K/Akt Signaling Pathway PMCID:    Pubmed No.:26346601 Efficient and sustained IGF-1 expression in the adipose tissue-derived stem cells mediated via a lentiviral vector PMCID:    Pubmed No.:25467975 Antidepressant effect of electroacupuncture regulates signal targeting in the brain and increases brain-derived neurotrophic factor levels PMCID:    Pubmed No.:27904490 Effect of Mechanism of Action of Different ω-6/ω-3 Polyunsaturated Fatty Acids Ratio on the Growth of Endometrial Carcinoma Mice PMCID:    Pubmed No.:25534488 Chronic unpredictable stress abrogates the endotoxin tolerance induced by repeated peripheral LPS challenge via the TLR4 signaling pathway PMCID:    Pubmed No.:28254553 Reduced β‑2‑glycoprotein І inhibits hypoxia‑induced retinal angiogenesis in neonatal mice through the vascular endothelial growth factor pathway PMCID:    Pubmed No.:25374014 17β-Estradiol Inhibites Tumor Necrosis Factor-α Induced Apoptosis of Human Nucleus Pulposus Cells via the PI3K/Akt Pathway PMCID:    Pubmed No.:27847386 Spatholobus suberectus Exhibits Antidiabetic Activity In Vitro and In Vivo through Activation of AKT-AMPK Pathway PMCID:    Pubmed No.:28607575 Urocortin ameliorates diabetic cardiomyopathy in rats via the Akt/GSK-3β signaling pathway PMCID:    Pubmed No.:25667611 Effect of bradykinin on TGF-β1-induced retinal pigment epithelial cell proliferation and extracellular matrix secretion PMCID:    Pubmed No.:27832751 Bergenin suppresses the growth of colorectal cancer cells by inhibiting PI3K/AKT/mTOR signaling pathway PMCID:    Pubmed No.:37259388 Osteosarcoma cells induce differentiation of mesenchymal stem cells into cancer associated fibroblasts through Notch and Akt signaling pathway PMCID:    Pubmed No.:31966700 Nicorandil inhibits oxidative stress and amyloid-β precursor protein processing in SH-SY5Y cells overexpressing APPsw PMCID:    Pubmed No.:25932125 Increased expression of EHF contributes to thyroid tumorigenesis through transcriptionally regulating HER2 and HER3 PMCID:    Pubmed No.:27517321 N-cadherin promotes thyroid tumorigenesis through modulating major signaling pathways PMCID:    Pubmed No.:28042956 Maternal butyrate supplementation induces insulin resistance associated with enhanced intramuscular fat deposition in the offspring PMCID:    Pubmed No.:28055958 MiR-221 Promotes Capan-2 Pancreatic Ductal Adenocarcinoma Cells Proliferation by Targeting PTEN-Akt PMCID:    Pubmed No.:27230035 TGF-β induces M2-like macrophage polarization via SNAIL-mediated suppression of a pro-inflammatory phenotype PMCID:    Pubmed No.:27418133 Inverse agonist of estrogen-related receptor α suppresses the growth of triple negative breast cancer cells through ROS generation and interaction with multiple cell signaling pathways PMCID:    Pubmed No.:26871469 SiRNA Directed Against Annexin II Receptor Inhibits Angiogenesis via Suppressing MMP2 and MMP9 Expression PMCID:    Pubmed No.:25633185 Inhibition of ERRα suppresses epithelial mesenchymal transition of triple negative breast cancer cells by directly targeting fibronectin PMCID:    Pubmed No.:26160845 Sulforaphane inhibits thyroid cancer cell growth and invasiveness through the reactive oxygen species-dependent pathway PMCID:    Pubmed No.:26312762 Afatinib reverses multidrug resistance in ovarian cancer via dually inhibiting ATP binding cassette subfamily B member 1 PMCID:    Pubmed No.:26317651 Tubb3 regulation by the Erk and Akt signaling pathways: a mechanism involved in the effect of arginine ADP-ribosyltransferase 1 (Art1) on apoptosis of colon carcinoma CT26 cells PMCID:    Pubmed No.:26373733 Osteopontin Promotes Invasion, Migration and Epithelial-Mesenchymal Transition of Human Endometrial Carcinoma Cell HEC-1A Through AKT and ERK1/2 Signaling PMCID:    Pubmed No.:26509489 Growth-stimulatory activity of TIMP-2 is mediated through c-Src activation followed by activation of FAK, PI3-kinase/AKT, and ERK1/2 independent of MMP inhibition in lung adenocarcinoma cells PMCID:    Pubmed No.:26556867 Interleukin-6 suppression reduces tumour self-seeding by circulating tumour cells in a human osteosarcoma nude mouse model PMCID:    Pubmed No.:26623559 miR-382 targeting PTEN-Akt axis promotes liver regeneration PMCID:    Pubmed No.:26636539 Frequent amplification of AIB1, a critical oncogene modulating major signaling pathways, is associated with poor survival in gastric cancer PMCID:    Pubmed No.:25970779 Structural analysis of Panax ginseng glycoproteins and its anti-oligoasthenozoospermia effect in vivo PMCID:    Pubmed No.:34743938 GPER-Induced ERK Signaling Decreases Cell Viability of Hepatocellular Carcinoma PMCID:    Pubmed No.:33767999 Adiponectin Attenuates Lipopolysaccharide-induced Apoptosis by Regulating the Cx43/PI3K/AKT Pathway PMCID:    Pubmed No.:34084134 The antimetastatic effect and underlying mechanisms of thioredoxin reductase inhibitor ethaselen PMCID:    Pubmed No.:30496814 Retinoic acid-induced 2 (RAI2) is a novel tumor suppressor, and promoter region methylation of RAI2 is a poor prognostic marker in colorectal cancer PMCID:    Pubmed No.:29796120 Metformin protects against insulin resistance induced by high uric acid in cardiomyocytes via AMPK signalling pathways in vitro and in vivo PMCID:    Pubmed No.:34053175 Transgenerational Inheritance of Betaine‐Induced Epigenetic Alterations in Estrogen‐Responsive IGF‐2/IGFBP2 Genes in Rat Hippocampus PMCID:    Pubmed No.:32022472 Butyrate Suppresses Glucose Metabolism of Colorectal Cancer Cells via GPR109a-AKT Signaling Pathway and Enhances Chemotherapy PMCID:    Pubmed No.:33855046 DNA Hypomethylation of GR Promoters is Associated with GR Activation and BDNF/AKT/ERK1/2‐Induced Hippocampal Neurogenesis in Mice Derived From Folic‐Acid‐Supplemented Dams PMCID:    Pubmed No.:30920123 2‐Methoxyestradiol attenuates chronic‐intermittent‐hypoxia‐induced pulmonary hypertension through regulating microRNA‐223 PMCID:    Pubmed No.:30246291 Increased Krüppel-like factor 12 impairs embryo attachment via downregulation of leukemia inhibitory factor in women with recurrent implantation failure PMCID:    Pubmed No.:30109142 Bisphenol A suppresses proliferation and induces apoptosis in colonic epithelial cells through mitochondrial and MAPK/AKT pathways PMCID:    Pubmed No.:30036487 YAP mediates the interaction between the Hippo and PI3K/Akt pathways in mesangial cell proliferation in diabetic nephropathy PMCID:    Pubmed No.:32816106 Lithium Posttreatment Alleviates Blood–Brain Barrier Injury After Intracerebral Hemorrhage in Rats PMCID:    Pubmed No.:29775701 Polysaccharides from Nostoc commune Vaucher activate macrophages via NF-κB and AKT/JNK1/2 pathways to suppress colorectal cancer growth in vivo PMCID:    Pubmed No.:31259992 Formononetin induces vasorelaxation in rat thoracic aorta via regulation of the PI3K/PTEN/Akt signaling pathway PMCID:    Pubmed No.:30464399 Let-7b regulates the adriamycin resistance of chronic myelogenous leukemia by targeting AURKB in K562/ADM cells PMCID:    Pubmed No.:32856506 Grandmaternal betaine supplementation enhances hepatic IGF2 expression in F2 rat offspring through modification of promoter DNA methylation PMCID:    Pubmed No.:31756772 The Protective Effect of Tetrahydroxystilbene Glucoside on High Glucose-Induced Injury in Human Umbilical Vein Endothelial Cells through the PI3K/Akt/eNOS Pathway and Regulation of Bcl-2/Bax PMCID:    Pubmed No.:34218226 Cross regulation of signaling pathways in gastrointestinal stromal tumor PMCID:    Pubmed No.:30405821 Follicle loss and PTEN/PI3K/mTOR signaling pathway activated in LepR-mutated mice PMCID:    Pubmed No.:30145913 Methyl Helicterate Inhibits Hepatic Stellate Cell Activation Through Modulation of Apoptosis and Autophagy PMCID:    Pubmed No.:30466104 Synthesis of dihydrofuran-3-one and 9,10-phenanthrenequinone hybrid molecules and biological evaluation against colon cancer cells as selective Akt kinase inhibitors PMCID:    Pubmed No.:35751771 Mechanism of Shiliu Buxue Syrup for anemia using integrated metabolomics and network pharmacology PMCID:    Pubmed No.:35690102 Adipose mesenchymal stem cell–derived exosomal microRNAs ameliorate polycystic ovary syndrome by protecting against metabolic disturbances PMCID:    Pubmed No.:35987860 Role of PI3K in the bone resorption of apical periodontitis PMCID:    Pubmed No.:35953782 Acteoside and ursolic acid synergistically protects H2O2-induced neurotrosis by regulation of AKT/mTOR signalling: from network pharmacology to experimental validation PMCID:    Pubmed No.:36102631 LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing PMCID:    Pubmed No.:36650118 γ-Glutamylcysteine rescues mice from TNBS-driven inflammatory bowel disease through regulating macrophages polarization PMCID:    Pubmed No.:36690783 A novel pharmaceutical preparation of Tripterygium wilfordii Hook. f. regulates macrophage polarization to alleviate inflammation in rheumatoid arthritis PMCID:    Pubmed No.:37738207
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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