Product Name :
HSL (phospho-S563) polyclonal antibody Background :
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Product :
PBS with 0.01% thiomersal,50% glycerol,pH7.3. Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
HSL (phospho-S563) polyclonal antibody detects endogenous levels of HSL (phospho-S563) protein. Immunogen :
A synthetic phosphorylated peptide around S563 of rat HSL (XP_006228456.1). Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot analysis of extracts of various cell lines, using HSL (phospho-S563) antibody at 1:1000 dilution.3T3L1 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.H9C2 cells were treated with Isoproterenol (10 μM for 20 min) at 37℃.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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