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IL10 polyclonal antibody BS80162
  • Immunofluorescence analysis of Jurkat cells using IL10 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Rat thymus using IL10 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product NameIL10 polyclonal antibody
Catalog No.BS80162
Swiss-ProtP22301
Host Rabbit
ReactivityHuman, Mouse, Rat
ApplicationsIF/ICC
Application_allIF/ICC,1:50 - 1:200
BiowMWRefer to figures
Alternative NameCSIF;GVHDS;IL-10;IL10A;TGIF;IL10
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $208
100ul $358
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Product Name :
IL10 polyclonal antibody
Background :
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Unmodification
Immunogen :
A synthetic peptide of human IL10(NP_000563.1).
Conjugate :
Unconjugated
Modification :
Unmodification
  • Immunofluorescence analysis of Jurkat cells using IL10 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Rat thymus using IL10 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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