Product Name :
Phospho-FoxO1-S256 polyclonal antibody Background :
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Phosphorylated Immunogen :
A synthetic phosphorylated peptide around S256 of human FoxO1(NP_002006.2). Conjugate :
Unconjugated Modification :
Phosphorylated

• 
  Western blot analysis of extracts of 293T cells, using Phospho-FoxO1-S256 antibody .293T were treated by CIP at 37℃ for 1 hour.
  Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
  Lysates/proteins: 25ug per lane.
  Blocking buffer: 3% BSA.
  Detection: ECL Basic Kit .
  Exposure time: 90s.
• 
  Western blot analysis of extracts of HeLa cells, using Phospho-FoxO1-S256 pAb at 1:1000 dilution or FOXO1 antibody .Hela cell lysate were treated by CIP at 37℃ for 1 hour or treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight..
  Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
  Lysates/proteins: 25ug per lane.
  Blocking buffer: 3% BSA.
  Detection: ECL Basic Kit .
  Exposure time: 2s.
• 
  Western blot analysis of extracts of HeLa cells, using Phospho-FoxO1-S256 pAb at 1:1000 dilution or FOXO1 antibody .Hela cell lysate were treated by CIP at 37℃ for 1 hour or treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight..
  Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
  Lysates/proteins: 25ug per lane.
  Blocking buffer: 3% BSA.
  Detection: ECL Basic Kit .
  Exposure time: 2s.
• 
  Western blot analysis of extracts of HeLa cells, using Phospho-FoxO1-S256 pAb at 1:1000 dilution or FOXO1 antibody .Hela cell lysate were treated by CIP at 37℃ for 1 hour or treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight..
  Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
  Lysates/proteins: 25ug per lane.
  Blocking buffer: 3% BSA.
  Detection: ECL Basic Kit .
  Exposure time: 2s.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---