Product Name :
SARS-CoV-2 Spike S2 ECD polyclonal antibody Background :
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Unmodification Immunogen :
A synthetic peptide of SARS-CoV-2 Spike S2 ECD(YP_009724390.1). Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of extracts of normal 293T cells 293T transfected with Spike S2 ECD Protein, using SARS-CoV-2 Spike S2 ECD antibody at 1:5000 dilution.
  Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
  Lysates/proteins: 25ug per lane.
  Blocking buffer: 3% nonfat dry milk in TBST.
  Detection: ECL Enhanced Kit .
  Exposure time: 1s.
• 
  Immobilized Recombinant SARS-COV2 S-ECD-PFS Protein at 1μg/mL can bind SARS-CoV-2 Spike S2 ECD Rabbit pAb with a linear range of 3.12-50ng/mL.
• 
  Immobilized Recombinant SARS-COV2 S-ECD-PFS Protein at 1μg/mL can bind SARS-CoV-2 Spike S2 ECD Rabbit pAb with a linear range of 3.12-50ng/mL.
• 
  Immobilized Recombinant SARS-COV2 S-ECD-PFS Protein at 1μg/mL can bind SARS-CoV-2 Spike S2 ECD Rabbit pAb with a linear range of 3.12-50ng/mL.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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