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DDX3 monoclonal antibody MB12352
  • Western blot analysis of DDX3 in COS7, Jurkat, C6, 3T3 and Hela lysates using DDX3 antibody.
Product NameDDX3 monoclonal antibody
Catalog No.MB12352
Swiss-ProtO00571
Host Mouse
ReactivityHuman,Rat,Mouse,Monkey
ApplicationsWB,IP,ICC/IF
Application_allWB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
BiowMWCalculated MW: 73 kDa; Observed MW: 73 kDa
Alternative NameDBX; DDX3; HLP2; DDX14.
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
DDX3 monoclonal antibody
Background :
Multifunctional ATP-dependent RNA helicase.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG2a
Immunogen :
Purified recombinant human DDX3 protein fragments expressed in E.coli.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of DDX3 in COS7, Jurkat, C6, 3T3 and Hela lysates using DDX3 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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