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SPT5 monoclonal antibody MB12256
  • Immunocytochemistry analysis of SPT5 in Hela using SPT5 antibody,and DAPI.
  • Western blot analysis of SPT5 in Hela, A549, HL-60 lysates using SPT5 antibody.
  • Western blot analysis of SPT5 in Hela, A549, HL-60 lysates using SPT5 antibody.
Product NameSPT5 monoclonal antibody
Catalog No.MB12256
Swiss-ProtO00267
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC-F,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 121 kDa; Observed MW: 150 kDa
Alternative NamehSPT5; SPT5; SPT5H; supt5h; Tat CT1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
SPT5 monoclonal antibody
Background :
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human SPT5
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunocytochemistry analysis of SPT5 in Hela using SPT5 antibody,and DAPI.
  • Western blot analysis of SPT5 in Hela, A549, HL-60 lysates using SPT5 antibody.
  • Western blot analysis of SPT5 in Hela, A549, HL-60 lysates using SPT5 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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