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SMARCA2 monoclonal antibody MB12250
  • Immunocytochemistry analysis of SMARCA2 in 293 using SMARCA2 antibody,and DAPI.
  • Western blot analysis of SMARCA2/BRM in A549, HL-60, U2OS lysates using SMARCA2/BRM antibody.
  • Western blot analysis of SMARCA2/BRM in A549, HL-60, U2OS lysates using SMARCA2/BRM antibody.
Product NameSMARCA2 monoclonal antibody
Catalog No.MB12250
Swiss-ProtP51531
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC-F,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 181 kDa; Observed MW: 190 kDa
Alternative NameSMARCA2; BAF190B; BRM; SNF2A; SNF2L2; Probable global transcription activator SNF2L2; ATP-dependent helicase SMARCA2; BRG1-associated factor 190B; BAF190B; Protein brahma homolog; hBRM; SNF2-alpha; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
SMARCA2 monoclonal antibody
Background :
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human SMARCA2/BRM
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunocytochemistry analysis of SMARCA2 in 293 using SMARCA2 antibody,and DAPI.
  • Western blot analysis of SMARCA2/BRM in A549, HL-60, U2OS lysates using SMARCA2/BRM antibody.
  • Western blot analysis of SMARCA2/BRM in A549, HL-60, U2OS lysates using SMARCA2/BRM antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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