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PF4 monoclonal antibody MB12189
  • Western blot analysis of PF4 in rat spleen lysates using PF4 antibody.
  • Western blot analysis of PF4 in A549, HL-60 lysates using PF4 antibody.
  • Western blot analysis of PF4 in A549, HL-60 lysates using PF4 antibody.
Product NamePF4 monoclonal antibody
Catalog No.MB12189
Swiss-ProtP02776
Host Rabbit
ReactivityHuman,Rat
ApplicationsWB,IHC-P,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
BiowMWCalculated MW: 11 kDa; Observed MW: 11 kDa
Alternative NameC-X-C motif chemokine 4; CXCL4; Iroplact; OncostatinA; PF4; Platelet factor 4; SCYB4; short form; Small inducible cytokine subfamily member 4
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
PF4 monoclonal antibody
Background :
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human PF4
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of PF4 in rat spleen lysates using PF4 antibody.
  • Western blot analysis of PF4 in A549, HL-60 lysates using PF4 antibody.
  • Western blot analysis of PF4 in A549, HL-60 lysates using PF4 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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