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Keap1 monoclonal antibody MB12091
  • Western blot analysis of Keap1 in Raw264.7 lysates using Keap1 antibody.
  • Western blot analysis of Keap1 in Hela, A549, C2C12 lysates using Keap1 antibody.
  • Western blot analysis of Keap1 in Hela, A549, C2C12 lysates using Keap1 antibody.
Product NameKeap1 monoclonal antibody
Catalog No.MB12091
Swiss-ProtQ14145
Host Rabbit
ReactivityHuman,Mouse
ApplicationsWB,IHC-P
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100
BiowMWCalculated MW: 70 kDa; Observed MW: 60-64 kDa
Alternative NameINrf2; KLHL19
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
Keap1 monoclonal antibody
Background :
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
Recombinant protein of human Keap1
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Keap1 in Raw264.7 lysates using Keap1 antibody.
  • Western blot analysis of Keap1 in Hela, A549, C2C12 lysates using Keap1 antibody.
  • Western blot analysis of Keap1 in Hela, A549, C2C12 lysates using Keap1 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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