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HIF Prolyl Hydroxylases monoclonal antibody MB12058
  • Western blot analysis of HIF Prolyl Hydroxylases in Hela, A549, HL-60, U251, U87-MG lysates using HIF Prolyl Hydroxylases antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human lung cancer using HIF Prolyl Hydroxylases antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NameHIF Prolyl Hydroxylases monoclonal antibody
Catalog No.MB12058
Swiss-ProtQ9NXG6
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC-P,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
BiowMWCalculated MW: 57 kDa; Observed MW: 43 kDa
Alternative NamePH4; PH-4; PHD4; EGLN4; HIDEA; HIFPH4; P4H-TM
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
HIF Prolyl Hydroxylases monoclonal antibody
Background :
The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human HIF Prolyl Hydroxylases
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of HIF Prolyl Hydroxylases in Hela, A549, HL-60, U251, U87-MG lysates using HIF Prolyl Hydroxylases antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human lung cancer using HIF Prolyl Hydroxylases antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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