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RNA Polymerase II Subunit B1 monoclonal antibody MB11834
  • Immunocytochemistry analysis of RNA Polymerase II Subunit B1 in CEM using RNA Polymerase II Subunit B1 antibody,and DAPI.
  • Western blot analysis of RNA polymerase II CTD repeat YSPTSPS in Hela, A549, MCF-7, U251, HL-60 lysates using RNA polymerase II CTD repeat YSPTSPS antibody.
  • Western blot analysis of RNA polymerase II CTD repeat YSPTSPS in Hela, A549, MCF-7, U251, HL-60 lysates using RNA polymerase II CTD repeat YSPTSPS antibody.
Product NameRNA Polymerase II Subunit B1 monoclonal antibody
Catalog No.MB11834
Swiss-ProtP24928
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC-F,IHC-P,ICC/IF,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
BiowMWCalculated MW: 217 kDa; Observed MW: 250 kDa
Alternative NamePOLR2A; POLR2; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
RNA Polymerase II Subunit B1 monoclonal antibody
Background :
During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human RNA polymerase II CTD repeat YSPTSPS
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunocytochemistry analysis of RNA Polymerase II Subunit B1 in CEM using RNA Polymerase II Subunit B1 antibody,and DAPI.
  • Western blot analysis of RNA polymerase II CTD repeat YSPTSPS in Hela, A549, MCF-7, U251, HL-60 lysates using RNA polymerase II CTD repeat YSPTSPS antibody.
  • Western blot analysis of RNA polymerase II CTD repeat YSPTSPS in Hela, A549, MCF-7, U251, HL-60 lysates using RNA polymerase II CTD repeat YSPTSPS antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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