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NEDD4 monoclonal antibody MB11823
  • Western blot analysis of NEDD4 in K562, HCT116, 3T3, C6 lysates using NEDD4 antibody.
  • Western blot analysis of NEDD4 in Hela, A549, HL-60, U2OS, U87-MG lysates using NEDD4 antibody.
Product NameNEDD4 monoclonal antibody
Catalog No.MB11823
Swiss-ProtP46934
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IP
Application_allWB: 1/500-1/1000 IP: 1/20
BiowMWCalculated MW: 149 kDa; Observed MW: 115 kDa
Alternative NameRPF1; NEDD4-1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
NEDD4 monoclonal antibody
Background :
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human NEDD4
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of NEDD4 in K562, HCT116, 3T3, C6 lysates using NEDD4 antibody.
  • Western blot analysis of NEDD4 in Hela, A549, HL-60, U2OS, U87-MG lysates using NEDD4 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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